glymotif vs Holistics

glymotif detects and counts structural motifs in glycans, over a built-in motif database or user-supplied structures, with fuzzy modification matching and alignment control. Database motifs are now requested through a db_motifs_spec object carrying their own matching parameters rather than as a name vector with loose arguments, and db_motif_info() exposes the built-in set as an inspectable tibble. A lenient mode lets lower-information glycans match more specific motifs while concrete mismatches still fail, and low-level entry points work directly on igraph objects for other package authors.

Performance has been a recurring line item across at least four releases, culminating in optimised graph searches and candidate filtering aimed at batch analyses, which points at the real workload being whole experiments rather than single glycans. The API has moved the other way from convenience toward explicitness: the add_motifs_lgl() and add_motifs_int() wrappers are deprecated in favour of composing with dplyr or glyexp verbs, optional arguments must now be named, and loose matching parameters were folded into the spec object. Documentation is being steered toward the cohort's newer container types, so this package is following a coordinated migration rather than setting its own course.

With the deprecated annotation wrappers on their way out and documentation already pointing at the replacement verbs, their removal is the likely next breaking change. The lenient matching mode is new enough that its boundary against concrete mismatches will probably need tuning as users apply it to real, partially resolved data.